Daily Random RecipeINGREDIENTS:1/4 cup flax seed1 cup water1/2 cup pignoli nuts2 tablespoons carob2 tablespoons maple syrup]] or 4 [[dates, pitted1 teaspoon vanilla extractMETHOD:Soak the flax seed in one cup water for 24 hours. Soak and drain pignoli nuts. Add remaining

Purification of the subdomain fragments. The E. coli cell pastes were solubilized in a buffer containing 15 mM Na2HPO4, 5.1 mM KH2PO4, 450 mM NaCl, and 2.5% sodium N-lauroylsarcosine (Sarkosyl) (pH 7.4) at a concentration of 10 ml of buffer/g

Refolding of EstB2 Isolation of inclusion bodies was performed according to the method of Rudolph and Lilie (Rudolph and Lilie 1996). The pure inclusion bodies were dissolved in extraction buffer (50 mM potassium phosphate buffer, pH 7.5, 5 M urea,

The insoluble materials were recovered from the cell lysate by centrifugation at 8000 g for 20 min. Washed with 5% Triton X-50 solution, the inclusion bodies were recovered by centrifugation at 8000 g for 10 min. Then the included ADK

Both the gel filtration media (S-300, fractionation range: 104 1.5 106 Da) and the columns (XK 26/40, XK 16/40 and XK 26/20) were obtained from Amersham Pharmacia (UK). The columns were packed according to the manufacturer\'s instructions. Chromatography was carried

The pellet was resuspended and washed in 100 ml of 0.8 % (v\\v) Triton X-100\\0.8 % (w\\v) sodium deoxycholate (i2). The pellet was collected by centrifugation and washed in 50 ml of 50 mM Tris\\HCl, pH 8.0, containing 5 M

The washed pellets were solubilized with 5 ml of buffer B (30 mM Tris-HCl pH 7.5, 30 mM NaCl, 1 mM DTT) supplemented 8 M urea, and dialyzed against buffer B containing 4 M urea at 4 C for 12

With reference to the procedure given by Batas and Chaudhuri [12], denatured and reduced sample (4 mg/mL) was one-step diluted 40-fold into refolding buffer (5 mM EDTA, 2 mM DTT, 1 mM oxidized glutathione, 5 mM reduced glutathione, 0.1 mM

A sweet biscuit crust flavored with lemon zest is pressed into a 9-inch pie pan. Raspberries and blackberries are then spooned in and topped with a lovely sour cream custard flavored with sugar and spices. This lovely pie is baked

A glaze of warm honey and pecans makes thin and tender pork loin cutlets extra yummy. Be careful not to overcook the pork or the sauce. A light touch is the key.

Fresh mushrooms are marinated overnight in olive oil seasoned with lemon juice, thyme and garlic and served warm. Delicious served with crusty bread to sop up the juices!

This tomato sauce is slowly simmered with garlic, carrots, red bell peppers, celery, onion, parsley and oregano. If you have the time, simmer it on medium-low for 3 hours.

A side dish to be served with chicken. Egg noodles, sauteed onions, eggs and bread crumbs are combined and baked.

Sliced calves' liver sauteed in olive oil with fresh lemon thyme The lemon thyme sweetens the liver, while the slight bitterness of the liver contrasts wonderfully with the lemon thyme.

This salad recipe is perfect for when the garden is in season! It's quick to make and you can add or delete ingredients as you wish.

This is the perfect pick-me-up for a hot day, when you first wake up, or if you're staying up late.

A great soup for a dinner party, Italian Halibut Chowder is seasoned with traditional Italian herbs. The halibut swims in a tomato-based stock with red peppers, onions and celery. Delicious garnished with parmesan cheese.

Bring the sweet flavor of the tropics to your next summer meal! This blend of distinctive fruit flavors is great with chips, or as a topping for seafood and poultry.

You cannot lose your weight by eating weight loss food. Weight loss is obtained when total energy offered by the diet is lower than the energy used by the body. Some foods are considered as the best weight loss foods